3g4 h00006201 m03 (Novus Biologicals)
Structured Review
3g4 H00006201 M03, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7/RPS7+Antibody+(3G4)/pmc12240844-116-29-27
Average 93 stars, based on 4 article reviews
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Incubation:Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome. Article Snippet: .. Molecular Psychiatry Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), ( Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), ( Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Article Snippet: The sections were then immersed in peroxidase blocking solution (DAKO, Carpinteria, CA, USA) for 10 min, followed by a 1 h incubation in protein block solution (DAKO, Carpinteria, CA, USA). .. The sections were then incubated overnight with primary antibodies from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:100), (RPL9, ab182556, 1:100), (RPS12, ab167428, 1:100), (RPS16, ab177951, 1:100), (RPS24, ab196652, 1:50), (HSP105, ab109624, 1:100), (IL-1β, ab9722, 1:100), (Bax, ab32503, 1:100), (Ki67, ab16667, 1:100), (Fibronectin, ab45688, 1:200)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:100), ( Article Title: Decreased expression of ribosomal protein Rpl3 contributes to behavioral deficits caused by Shank3 deficiency Article Snippet: Free- oating brain coronal sections were blocked in 3% bovine serum albumin (neoFroxx, Einhausen, Germany) and 0.3% Triton (Roche, Basel, Switzerland) in PBS w/o Ca2+ and Mg2+ for 2 hours at room temperature (RT). .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 hours at 4°C Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome Article Snippet: For Shank3 staining a blocking solution of 10% goat serum, 5% FBS and 0.1% tryton in PBS3% w/o Ca 2+ and Mg 2+ was used. .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Blocking Assay:Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome. Article Snippet: .. Molecular Psychiatry Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. Article Title: Decreased expression of ribosomal protein Rpl3 contributes to behavioral deficits caused by Shank3 deficiency Article Snippet: Free- oating brain coronal sections were blocked in 3% bovine serum albumin (neoFroxx, Einhausen, Germany) and 0.3% Triton (Roche, Basel, Switzerland) in PBS w/o Ca2+ and Mg2+ for 2 hours at room temperature (RT). .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 hours at 4°C Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome Article Snippet: For Shank3 staining a blocking solution of 10% goat serum, 5% FBS and 0.1% tryton in PBS3% w/o Ca 2+ and Mg 2+ was used. .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Western Blot:Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), ( Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), ( Immunohistochemistry:Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Article Snippet: The sections were then immersed in peroxidase blocking solution (DAKO, Carpinteria, CA, USA) for 10 min, followed by a 1 h incubation in protein block solution (DAKO, Carpinteria, CA, USA). .. The sections were then incubated overnight with primary antibodies from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:100), (RPL9, ab182556, 1:100), (RPS12, ab167428, 1:100), (RPS16, ab177951, 1:100), (RPS24, ab196652, 1:50), (HSP105, ab109624, 1:100), (IL-1β, ab9722, 1:100), (Bax, ab32503, 1:100), (Ki67, ab16667, 1:100), (Fibronectin, ab45688, 1:200)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:100), ( Ubiquitin Proteomics:Article Title: LncRNA NRON promotes tumorigenesis by enhancing MDM2 activity toward tumor suppressor substrates. Article Snippet: The E3 ligase MDM2 promotes tumor growth and progression by inducing ubiquitin-mediated degradation of P53 and other tumorsuppressing proteins.. Here, we identified an MDM2-interacting lncRNA NRON, which promotes tumor formation by suppressing both P53-dependent and independent pathways.. NRON binds to MDM2 and MDMX (MDM4) via two different stem-loops, respectively, and induces their heterogenous dimerization, thereby enhancing the E3 ligase activity of MDM2 toward its tumorsuppressing substrates, including P53, RB1, and NFAT1. |
