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3g4 h00006201 m03  (Novus Biologicals)


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    Structured Review

    Novus Biologicals 3g4 h00006201 m03
    3g4 H00006201 M03, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rps7/RPS7+Antibody+(3G4)/pmc12240844-116-29-27
    Average 93 stars, based on 4 article reviews
    3g4 h00006201 m03 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome.
    Article Snippet: .. Molecular Psychiatry Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP220214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100; Shank3, rabbit, Cell Signaling, cat. 64555, 1:500; Synapsin1, mouse, Synaptic System, cat. 106 011, 1:500. ..

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement
    Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), (RPS7, PA5-110326, 1:1000)], from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:1000)] and from Cell Signaling Technology (Danvers, MA USA) [β-actin, 4970, 1:1000), (GAPDH, 2118, 1:1000) followed by incubation with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000; Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. An ECL Plus Western Blotting Detection System (GE Healthcare, Tokyo, Japan) was used to visualize the immunoreactive bands, and images were captured using the Luminescent ImageQuant LAS 4000 Mini (GE Healthcare, Tokyo, Japan). ..

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.
    Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), (RPS7, PA5-110326, 1:1000)], from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:1000)] and from Cell Signaling Technology (Danvers, MA USA) [β-actin, 4970, 1:1000), (GAPDH, 2118, 1:1000) followed by incubation with horseradish peroxidaseconjugated anti-rabbit IgG (1:2000; Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. An ECL Plus Western Blotting Detection System (GE Healthcare, Tokyo, Japan) was used to visualize the immunoreactive bands, and images were captured using the Luminescent ImageQuant LAS 4000 Mini (GE Healthcare, Tokyo, Japan). ..

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.
    Article Snippet: The sections were then immersed in peroxidase blocking solution (DAKO, Carpinteria, CA, USA) for 10 min, followed by a 1 h incubation in protein block solution (DAKO, Carpinteria, CA, USA). .. The sections were then incubated overnight with primary antibodies from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:100), (RPL9, ab182556, 1:100), (RPS12, ab167428, 1:100), (RPS16, ab177951, 1:100), (RPS24, ab196652, 1:50), (HSP105, ab109624, 1:100), (IL-1β, ab9722, 1:100), (Bax, ab32503, 1:100), (Ki67, ab16667, 1:100), (Fibronectin, ab45688, 1:200)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:100), (RPS7, PA5-110326, 1:150)], and from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:100)], followed by incubation with appropriate secondary antibodies (MAX-PO, Nichirei Biosciences Inc., Tokyo, Japan) at room temperature for 30 min. For immunohistochemistry, diaminobenzidine (DAB) solution (DAKO, Carpinteria, CA, USA) was employed to develop color, and hematoxylin counterstaining was performed. .. An optical microscope (DP74; Olympus, Tokyo, Japan) was used to obtain the images.

    Article Title: Decreased expression of ribosomal protein Rpl3 contributes to behavioral deficits caused by Shank3 deficiency
    Article Snippet: Free- oating brain coronal sections were blocked in 3% bovine serum albumin (neoFroxx, Einhausen, Germany) and 0.3% Triton (Roche, Basel, Switzerland) in PBS w/o Ca2+ and Mg2+ for 2 hours at room temperature (RT). .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 hours at 4°C Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100. .. The day after, after 3 washes in PBS, brain sections were incubated with the secondary antibodies (STAR RED anti-mouse, 1:100, STRED-1001-500UG, Aberrior; STAR ORANGE anti-rabbit, 1:100, STORANGE-1002500UG, Aberrior) in blocking solution for 2 hours at RT.

    Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome
    Article Snippet: For Shank3 staining a blocking solution of 10% goat serum, 5% FBS and 0.1% tryton in PBS3% w/o Ca 2+ and Mg 2+ was used. .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100; Shank3, rabbit, Cell Signaling, cat. 64555, 1:500; Synapsin1, mouse, Synaptic System, cat. 106 011, 1:500. ..

    Blocking Assay:

    Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome.
    Article Snippet: .. Molecular Psychiatry Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP220214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100; Shank3, rabbit, Cell Signaling, cat. 64555, 1:500; Synapsin1, mouse, Synaptic System, cat. 106 011, 1:500. ..

    Article Title: Decreased expression of ribosomal protein Rpl3 contributes to behavioral deficits caused by Shank3 deficiency
    Article Snippet: Free- oating brain coronal sections were blocked in 3% bovine serum albumin (neoFroxx, Einhausen, Germany) and 0.3% Triton (Roche, Basel, Switzerland) in PBS w/o Ca2+ and Mg2+ for 2 hours at room temperature (RT). .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 hours at 4°C Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100. .. The day after, after 3 washes in PBS, brain sections were incubated with the secondary antibodies (STAR RED anti-mouse, 1:100, STRED-1001-500UG, Aberrior; STAR ORANGE anti-rabbit, 1:100, STORANGE-1002500UG, Aberrior) in blocking solution for 2 hours at RT.

    Article Title: Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome
    Article Snippet: For Shank3 staining a blocking solution of 10% goat serum, 5% FBS and 0.1% tryton in PBS3% w/o Ca 2+ and Mg 2+ was used. .. Slices were thereafter incubated with the following primary antibodies diluted in blocking solution for 24 h at 4 °C: Rpl3, rabbit, Novus, cat. NBP2-20214, 1:500; Rps7, mouse, Novus Cat. (3G4) H00006201-M03, 1:100; Shank3, rabbit, Cell Signaling, cat. 64555, 1:500; Synapsin1, mouse, Synaptic System, cat. 106 011, 1:500. ..

    Western Blot:

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement
    Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), (RPS7, PA5-110326, 1:1000)], from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:1000)] and from Cell Signaling Technology (Danvers, MA USA) [β-actin, 4970, 1:1000), (GAPDH, 2118, 1:1000) followed by incubation with horseradish peroxidase-conjugated anti-rabbit IgG (1:2000; Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. An ECL Plus Western Blotting Detection System (GE Healthcare, Tokyo, Japan) was used to visualize the immunoreactive bands, and images were captured using the Luminescent ImageQuant LAS 4000 Mini (GE Healthcare, Tokyo, Japan). ..

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.
    Article Snippet: .. The membranes were incubated overnight with primary antibodies: from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:1000), (RPL9, ab182556, 1:2000), (RPS12, ab167428, 1:1000), (RPS16, ab177951, 1:1000), (RPS24, ab196052, 1:1000), (HSP105, ab109624, 1:1000), (Bax ab32503, 1:1000), (HIF-1α, ab2185, 1:500)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:1000), (RPS7, PA5-110326, 1:1000)], from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:1000)] and from Cell Signaling Technology (Danvers, MA USA) [β-actin, 4970, 1:1000), (GAPDH, 2118, 1:1000) followed by incubation with horseradish peroxidaseconjugated anti-rabbit IgG (1:2000; Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. An ECL Plus Western Blotting Detection System (GE Healthcare, Tokyo, Japan) was used to visualize the immunoreactive bands, and images were captured using the Luminescent ImageQuant LAS 4000 Mini (GE Healthcare, Tokyo, Japan). ..

    Immunohistochemistry:

    Article Title: Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement.
    Article Snippet: The sections were then immersed in peroxidase blocking solution (DAKO, Carpinteria, CA, USA) for 10 min, followed by a 1 h incubation in protein block solution (DAKO, Carpinteria, CA, USA). .. The sections were then incubated overnight with primary antibodies from Abcam (Cambridge, UK) [(RPL4, ab174269, 1:100), (RPL9, ab182556, 1:100), (RPS12, ab167428, 1:100), (RPS16, ab177951, 1:100), (RPS24, ab196652, 1:50), (HSP105, ab109624, 1:100), (IL-1β, ab9722, 1:100), (Bax, ab32503, 1:100), (Ki67, ab16667, 1:100), (Fibronectin, ab45688, 1:200)], from Thermo Fisher Scientific (Waltham, MA, USA) [(RPL6, PA5-30217, 1:100), (RPS7, PA5-110326, 1:150)], and from Novus Biologicals (Centennial, CO, USA) [(Dec1, NB100-1800, 1:100)], followed by incubation with appropriate secondary antibodies (MAX-PO, Nichirei Biosciences Inc., Tokyo, Japan) at room temperature for 30 min. For immunohistochemistry, diaminobenzidine (DAB) solution (DAKO, Carpinteria, CA, USA) was employed to develop color, and hematoxylin counterstaining was performed. .. An optical microscope (DP74; Olympus, Tokyo, Japan) was used to obtain the images.

    Ubiquitin Proteomics:

    Article Title: LncRNA NRON promotes tumorigenesis by enhancing MDM2 activity toward tumor suppressor substrates.
    Article Snippet: The E3 ligase MDM2 promotes tumor growth and progression by inducing ubiquitin-mediated degradation of P53 and other tumorsuppressing proteins.. Here, we identified an MDM2-interacting lncRNA NRON, which promotes tumor formation by suppressing both P53-dependent and independent pathways.. NRON binds to MDM2 and MDMX (MDM4) via two different stem-loops, respectively, and induces their heterogenous dimerization, thereby enhancing the E3 ligase activity of MDM2 toward its tumorsuppressing substrates, including P53, RB1, and NFAT1.



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    Image Search Results


    Plasma EV protein biomarkers for the diagnosis of LUSC. ( A ) Proteins with the highest predictive values in classifying LUSC and BLD with XGBoost. ( B ) ROC curve and confusion matrix in the training set. ( C ) ROC curve and confusion matrix in the test set. ( D ) ROC curve and confusion matrix in the entire cohort. SEN (Sensitivity) and SPE (Specificity) refer to diagnostic performance metrics for distinguishing LUSC from BLD samples. ( E ) Levels of TUBB3 in total plasma from patients with LUSC and BLD. A total of 74 samples (38 BLD and 36 LUSC) were tested. ( F ) Levels of RPS7 in total plasma from patients with LUSC and BLD. A total of 149 samples (44 BLD and 105 LUSC) were tested

    Journal: Cancer Cell International

    Article Title: Liquid biopsy-derived extracellular vesicle protein biomarkers for diagnosis and prognostic assessment of lung squamous cell carcinoma

    doi: 10.1186/s12935-025-03792-0

    Figure Lengend Snippet: Plasma EV protein biomarkers for the diagnosis of LUSC. ( A ) Proteins with the highest predictive values in classifying LUSC and BLD with XGBoost. ( B ) ROC curve and confusion matrix in the training set. ( C ) ROC curve and confusion matrix in the test set. ( D ) ROC curve and confusion matrix in the entire cohort. SEN (Sensitivity) and SPE (Specificity) refer to diagnostic performance metrics for distinguishing LUSC from BLD samples. ( E ) Levels of TUBB3 in total plasma from patients with LUSC and BLD. A total of 74 samples (38 BLD and 36 LUSC) were tested. ( F ) Levels of RPS7 in total plasma from patients with LUSC and BLD. A total of 149 samples (44 BLD and 105 LUSC) were tested

    Article Snippet: Following the manufacturer’s instructions, commercial assay kits for RPS7 (MyBioSource, USA, Catalog Number: MBS7204349) and TUBB3 (CUSABIO, China, Catalog Number: CSB-E14121h) were used to determine the expression levels of these proteins in preoperative plasma samples.

    Techniques: Clinical Proteomics, Biomarker Discovery, Diagnostic Assay